猪SOCS3基因的克隆、生物信息分析及热应激条件下的表达

doi:10.11843/j.issn.0366-6964.2014.07.003

Abstract

Abstract:

Suppressor of cytokine signaling 3 (SOCS3) associated with a lot of auto-immune diseases,so it was speculated that it might play an important role in the pathological process of heat stress in pigs.The porcine SOCS3 gene was cloned in this study，it consisted of 690 bp of ORF encoding a protein precursor with 229 amino acids，which shared high amino acid sequence identity with that of Bamei pig (98.2%)，Equus caballus (97.4%)，Bos Taurus (96.5%)，Homo sapiens (97.8%) and Mus musculus (96.4%).The molecular weight of SOCS3 protein was 25.1 ku，and PI was 8.84.It also had the classic structural trait of SOCS family，in which a central SH2 domain，a C-terminal 40-amino-acid SOCS box domain and a 12-amino-acid KIR domain were consisted.The dynamic of SOCS3 mRNA in thymus gland，spleen，mesenteric lymph node and intestine lamina propria of pigs during 10 days consecutive heat stress was investigated.The expression of SOCS3 in mesenteric lymph node were decreased on day 3，6 and 9 after heat stress begin significantly（P≤0.05），but a drastic up-regulation on day 6 and 9 in the spleen and intestine lamina propria，although a reduced tendency was displayed on day 3（P≤0.05）.The porcine SOCS3 cDNA was cloned successfully，and a significant changes of SOCS3 mRNA expression was investigated in heat stressed pigs with a tissue and time dependence manner.

CLC Number:

S828
S813.3

WANG Ping，JU Xiang-hong，YONG Yan-hong,JIA Ru-min，ZHAO Yun-tao,MA Ming-long，LIAO Ming. Cloning and Bioinformatics Analysis of Porcine SOCS3 cDNA and Its Expression Pattern during Heat Stress[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1044-1052.

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Cloning and Bioinformatics Analysis of Porcine SOCS3 cDNA and Its Expression Pattern during Heat Stress

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